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PAMPA Assay

PAMPA Assay

At Aurigene Pharmaceutical Services, our ADME services help understand the permeability of a drug by using Parallel Artificial Membrane Permeability (PAMPA) assay, an artificial lipid-infused assay used to measure the passive and transcellular permeability of a test compound. The advantage of using PAMPA assay over other assays is the capability to evaluate permeability over a large spectrum of pH ranges in a short period of time.

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Advantages of our Parallel Artificial Membrane Permeability (PAMPA) assay

  • Quick turn around time
  • Broad pH range
  • High (Dimethyl sulfoxide) DMSO content
  • High throughput (96-well plate assay)

PAMPA eliminates the interference of active transporters or efflux systems present in other assays, allowing the compound to be measured based on permeability data alone.

Test Article Concentration 10µM
Number of Replicates 3
Membrane Composition Precoated (lipid-infused artificial membrane)
Incubation Time 5 hours
Temperature 25 °C
Test Article Requirements 50µL (10mM DMSO stock)
Integrity Marker Not applicable (Standard quality controls)
Analysis Method LCMS-MS
Data Delivery 5 working days

PAMPA assay provides researchers a quick, economical method of evaluating the permeability of New Chemical Entities (NCEs).

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Our team of experts is dedicated to providing personalised solutions tailored to your unique needs. So, please don't hesitate to reach out to us. We look forward to hearing from you and helping you achieve your business goals.

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