Our team has experience in handling difficult and complex target molecules. We offer wide range of services and solutions with the flexibility of multiple entry points. We are proficient with most modern expression platforms and our facilities are equipped to handle scales ranging from mgs to grams.
Protein crystallography services provide a one-stop solution for determining 3D structure of: Proteins & complexes : Protein-protein complexes Protein-small molecule complexes Antibody-antigen complexes at high resolution
Protein crystallography projects are typically carried out in gene-to-structure format encompassing gene cloning, protein expression, purification, crystallization, screening, synchrotron x-ray diffraction, structure solution, and refinement.
Our structural biology expertise also includes protein engineering to enhance crystallizability of difficult proteins. We seamlessly integrate structural biology with drug discovery programs for end-to-end SBDD offerings.
Expression systems
Mammalian: Mammalian systems used for expression are CHO, ExpiCHO, HEK, ExpiHEK, etc. Protein production in transient expression mode or using stable clones or pools.
Insect cell: Baculovirus mediated protein expression and production in Sf9, Sf21, Hi5 and super Sf9 insect cell lines
Yeast: Stable protein expression in Pichia, recombinant molecule is typically secreted in media by genome integration.
Bacterial: Soluble, periplasmic and inclusion bodies in E. coli. Multi-protein expression by co-transfection or duet plasmid technology. Refolding condition screening and method optimization. Construct design, scouting and screening studies for challenging proteins. Protein expression scale-up using spinner flask, shaker flask, HYPER flask and WAVE bioreactor platforms to handle volumes up to 50 L scale with low operating costs
Protein Purification
Combination of high-end instruments and a wealth of scientific expertise we provide the best in class customized purification platforms for research and drug discovery needs. We are proficient in purifying tagged as well as un-tagged and all classes of target proteins from mammalian, yeast and microbial expression systems.
- Purification process optimization
- Protein purification in batch binding mode as well as automated FPLC systems
- Chromatographic purification methods include IMAC, IEC, mixed-mode, HIC, SEC and affinity for micrograms to gram-scale quantities
Antibody purification
- Purification of monoclonal antibodies of different origins (Human, non-human Primates, Mouse, Rat, Rabbit, Pig, Hamster, Gerbil, etc.)
- Purification of antibody fragments of various formats (Fab, Fab2, F(ab)’2, VHH, scfv and generation from full length mAbs
- Bi-specific and KIH antibodies
- Diabodies
- Single domain antibody
- Protein purification from hybridoma cultures
Microbial purification capabilities
- Soluble protein
- Periplasmic protein
- Intracellular protein
- Refolding from inclusion bodies
- Purification of Major Histocompatibility (MHC) complexes
- Membrane protein production – GPCRs, nanodisc technology, etc.
- Antibody drug conjugate screening, development and purification
- High throughput protein screening and purification
- Protein cleavage and purification
Protein Analytics and Characterization
We are committed to deliver high-quality products by performing a number of routine protein analyses throughout all project stages ensuring the identity, purity, structural and conformational integrity, stability and function of the protein.
- Quantitative analysis: Protein estimation using Absorbance at 280nm, Bradford and BCA assays
- Qualitative analysis: SDS-PAGE (Reducing/Non-reducing) and DOT/Western blot analysis
- Purity: SE-HPLC, IEX-HPLC and RP-HPLC
- Protein stability analysis (Real-time and Accelerated Stability Testing)
- LC-MS analysis
- Capillary electrophoresis (CE)
- Peptide Mass Fingerprinting (PMF)
- Biological activity and binding studies using enzymatic assays, ELISA, Biacore, etc.
- Endotoxin analysis
- Host cell protein (HCP) and Host cell DNA (HCD) analysis
Protein Stability Analysis
- Real-time and accelerated stability testing.
- Long-term stability studies.
Protein conjugation and labeling
- Protein labeling/conjugation (HRP, biotin, Ruthenium, fluorescent labels, etc.)
- Conjugation of resin with specific protein/peptide
Crystallization screening
- Multi-dimensional optimization of crystallization conditions using commercial and customized screens for a target protein or complex
- Sitting drop, hanging drop, micro-batch (under oil), and seeding
- micro-seeding and macro-seeding
Crystal hit identification
- Microscopic examination of drops, staining, and mass spectromety of harvested crystals
- Preliminary X-ray diffraction
Protein-protein and protein-drug complexes
- Crystallization of protein-inhibitor/ligand complexes (co-crystallization)
- Soaking of crystals in inhibitor/ligand solution
Data collection and refinement
- Cryoprotectant screening
- Complete X-ray data collection using rotating anode source
- Synchrotron data collection at Australian synchrotron
- Molecular replacement and structure refinement
- Structure analysis and data presentation
Why Aurigene Protein Sciences and Structural Biology Services?
Multidisciplinary team of scientists
Scalability with high purity and exceptional quality
Processing complex molecules
Protein engineering for enhanced crystallizability
Synchrotron data collection
Virtual Tour
Hyderabad R and D Center
Bangalore R and D Center
Connect with our scientific experts for your drug discovery, development, and manufacturing needs
We understand that clear communication is essential to successful collaborations, and that's why we have a dedicated team that is always ready to help you. Whether you have questions about our services, want to discuss a potential partnership, or simply want to learn more about our company, we're here to help.
Our team of experts is dedicated to providing personalised solutions tailored to your unique needs. So, please don't hesitate to reach out to us. We look forward to hearing from you and helping you achieve your business goals.
Resources
OCTOBER 01, 2024
PROTACs: Research for a life without cancer
PROTACs: Proteolysis-targeting chimeras (PROTACs) are a rapidly evolving field with promising applications in cancer, neurodegenerative diseases, and other conditions where the regulation of protein levels is crucial. PROTACs are a novel class of small molecules designed to target specific proteins for degradation by the ubiquitin-proteasome sys...
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Advancement in personalized medicine and how the CRDMO industry is part of the solution
Personalized medicine is transforming the healthcare landscape by customizing treatment plans to individual patients’ unique genetic, clinical and environmental characteristics. These are effective and less invasive treatments for a wide range of conditions. Contract Research, Development and Manufacturing Organizations (CRDMOs) play an important role...
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Cell Line Development
We enable development of stable and high yielding recombinant Mammalian and Microbial lines. ...
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Case study: Tackling CYP 2C9 inhibition challenges
The Problem: Active compounds in a project were found to be highly potent inhibitors of CYP 2C9 The compounds selectively inhibited CYP 2C9 with IC50 values <100 nM There was no considerable inhibition of the other CYP isoforms Our Mitigation Approach: CYP 2C9 inhibition data was generated for a larger set of co...
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Synthesis of Anti-covid Drug Nirmatrelvir Using Flow Chemistry
2022
Synthesis of the anti-covid therapeutic Nirmatrelvir by using flow chemistry to enhance efficiency of amide to nitrile conversion in a functionally and Stereochemically Embellished environment. ...
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Discovery of MAP855, an Efficacious and Selective MEK1/2 Inhibitor with an ATP-Competitive Mode of Action.
2005
Mutations in MEK1/2 have been described as a resistance mechanism to BRAF/MEK inhibitor treatment. We report the discovery of a novel ATP-competitive MEK1/2 inhibitor with efficacy in wildtype (WT) and mutant MEK12 models. Starting from a HTS hit, we obtained selective, cellularly active ...
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Wang-OSO3H catalyzed green synthesis of bioactive isoindolo[2,1- a ]quinazoline-5,11–dione derivatives: An unexpected observation
2005
The sulphonic acid-functionalized Wang resin (Wang-OSO3H) was explored as a polymeric and recov- erable acidic catalyst for the synthesis of isoindolo[2,1- a ]quinazoline-5,11–dione derivatives under green conditions. Thus the Wang-OSO3H ...
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Polycyclic Aromatic Compounds: A Simple and Efficient [(n-Bu3Sn)2MO4]n Catalyzed Synthesis of Quinazolinones and Dihydroquinazolinones
2005
A novel unprecedented approach for the synthesis of various quinazolinones and dihydroquinazolinones has been using [(n-Bu3Sn)2MO4]n as a catalyst. The reaction has been screened ...
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