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In vitro Biology In vitro Biology

In vitro Biology

In vitro biology services at Aurigene are focused on running standard and highly specialized biochemical and cell based assays and providing expertise to support stand alone or Integrated Drug Discovery programs.

Our in vitro biology team is highly skilled in wide range of therapeutic areas and target classes. De novo assay development for novel target classes or therapeutic indications, as well as assay customization, has added significant value to our Integrated Drug Discovery (IDD) projects. This has also resulted in successful compound progression and clinical candidate nomination.

Our offerings include screening and profiling services or platforms under different therapeutic areas and target classes.

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In vitro assay Capabilities

A platform for screening of NCEs and NBEs against major therapeutic target classes:

GPCRs, ion channels, proteases, other hydrolytic enzymes, and membrane receptors are a few examples of our experience in target classes.

APSL focuses and caters to a wide range of therapeutic areas like asthma and COPD, metabolic disorders (diabetes, NASH & obesity), inflammation, oncology, immunology, stem cell biology, osteoporosis, angiogenesis, CNS, and pain. We also have experience in epigenetics and Protein-Protein Interaction (PPI) targets.

At APSL, we are continuously adding the services for advanced and emerging drug classes like PROTAC, Oligonucleotide, ADC, and mRNA. We offer a complete set of bioassays for PROTAC discovery.

In vitro assay Development

  • Phenotypic assays:   Phenotypic assays aim to identify the molecules which can alter a cell's phenotype. Here, we look at the effect of a substance on the entire biological system as opposed to an isolated component of it. Phenotypic Assays are the most refined and delicate of all assays and can be performed in cells, such as stem-cell-derived human cells, isolated tissues, or entire organisms.
  • Mechanistic assays:   We have the core expertise for the level of mechanistic understanding required for drug discovery. Mechanistic assays are an important part of our in vitro service portfolio, as they provide further information about the cell mechanism displayed by the study compound. All assays are with fluorescence or luminescence endpoints.
  • Cell-based assays:   To understand the correlation of networks in a complex biological system, we can design custom cell-based assays that cater to every stage of drug development. As a leading CDMO, we have at our disposal a wide range of cell types which in combination with innovative technologies result in assays with improved translational success.
  • Readout Platforms:   We offer diverse readout platforms such as Luminescence, Fluorescence (FRET, TR-FRET), Fluorescence Polarization (FP), AlphaLISA, AlphaScreen, Absorbance, and UV to NIR.

In vitro assay Capability Range

Functional Assay

  • Cell Viability
  • Apoptosis
  • Invasion
  • Migration
  • Cell Senescence
  • Cell Cycle Analysis
  • Necroptosis Assay
  • Pyroptosis Assay

Specialized Assays

  • Target engagement assays via Nano-BRET/ CESTA
  • 3D cells co-culture
  • Spheroid culture
  • Organoid culture
  • Stem cell-derived functional cells – Hepatocytes, Cardiac, etc

Immunological Assays

  • Dendritic cell
  • T-cell cytotoxicity
  • Treg – Immune suppression
  • Immune cell profiling
  • Mixed lymphocyte

Biomarker Assay

  • Cytokine modulation
  • Target gene expression
  • Phosphorylation of signaling intermediates
  • Cytokine-induced transcription factor phosphorylation

Why Aurigene In vitro Biology Services?

Complex and specialized assay development

High throughput expression system

Functional, Mechanism of Action (MoA) and target engagement studies

Other Services

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Connect with our scientific experts for your drug discovery, development, and manufacturing needs

We understand that clear communication is essential to successful collaborations, and that's why we have a dedicated team that is always ready to help you. Whether you have questions about our services, want to discuss a potential partnership, or simply want to learn more about our company, we're here to help.

Our team of experts is dedicated to providing personalised solutions tailored to your unique needs. So, please don't hesitate to reach out to us. We look forward to hearing from you and helping you achieve your business goals.

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PROTACs: Research for a life without cancer

OCTOBER 01, 2024

PROTACs: Research for a life without cancer

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