Gene to Protein

Designed to meet scale and fit-for-purpose quality criteria of tool and therapeutic molecules.

Our team has experience in handling difficult and complex target molecules. We offer wide range of services and solutions with the flexibility of multiple entry points. We are proficient with most modern expression platforms and our facilities are equipped to handle scales ranging from milligrams to grams.

Membrane proteins: Our specialized expertise in recombinantly expressing and purifying integral membrane proteins enables their use in high-throughput assays and as antigens for immunization programs.

Protein crystallography services provide a one-stop solution for determining 3D structure of proteins and complexes:Protein-protein complexes Protein-small molecule complexes Antibody-antigen complexes at high resolution

Protein crystallography projects can be carried out in:

Gene-to-structure format: Gene cloning, protein expression, purification, crystallization, screening, synchrotron x-ray diffraction, structure solution and refinement.

Protein-to-structure format: Client supplied protein, quality checked in-house, crystallization screening, synchrotron x-ray diffraction, structure solution and refinement.

Standalone structure refinement: Crystal structure refinement for publication or uploading to protein data banks on client’s behalf.

Our structural biology expertise also includes empirical protein design to enhance crystallizability of difficult proteins. We seamlessly integrate structural biology with drug discovery programs for end-to-end SBDD offerings.

Speak to our experts

Expression Systems

Mammalian: Mammalian systems used for expression are CHO, ExpiCHO, HEK, ExpiHEK, etc. Protein production in transient expression mode or using stable clones or pools.

Insect cell: Baculovirus mediated protein expression and production in Sf9, Sf21, Hi5, and super Sf9 insect cell lines

Yeast: Stable protein expression in Pichia, recombinant molecule is typically secreted in media by genome integration.

Bacterial: Soluble, and inclusion bodies in E. coli. Multi-protein expression by co-transfection or duet plasmid technology. Refolding condition screening and method optimization. Construct design, scouting, and screening studies for challenging proteins. Protein expression scale-up using spinner flask, shaker flask, HYPER flask, and WAVE bioreactor platforms to handle volumes up to 50 L scale with low operating costs.

Protein Purification

With a combination of high-end instruments and a wealth of scientific expertise, we provide the best-in-class customized purification platforms for research and drug discovery needs. We are proficient in purifying tagged as well as un-tagged and all classes of target proteins from mammalian, yeast, and microbial expression systems.

Purification process optimization
Protein purification in batch binding mode as well as automated FPLC systems
Chromatographic purification methods include IMAC, IEC, mixed-mode, HIC, SEC, and affinity for micrograms to gram-scale quantities

Antibody Purification

Purification of monoclonal antibodies of different origins (human, non-human primates, mouse, rat, rabbit, pig, hamster, gerbil, etc.)
Purification of antibody fragments of various formats (Fab, Fab2, F(ab)’2, VHH, scfv, and generation from full length mAbs)
Bi-specific and KIH antibodies
Diabodies
Single domain antibody
Protein purification from hybridoma cultures

Microbial Purification Capabilities

Soluble protein
Periplasmic protein
Intracellular protein
Refolding from inclusion bodies
Purification of Major Histocompatibility (MHC) complexes
Antibody drug conjugate screening, development and purification
High throughput protein screening and purification
Protein cleavage and purification

Protein Analytics and Characterization

We are committed to deliver high-quality products by performing a number of routine protein analyses throughout all project stages ensuring the identity, purity, structural and conformational integrity, stability and function of the protein.

Quantitative analysis: Protein estimation using Absorbance at 280nm, Bradford and BCA assays
Qualitative analysis: SDS-PAGE (Reducing/Non-reducing) and DOT/Western blot analysis
Purity: SE-HPLC, IEX-HPLC and RP-HPLC
Protein stability analysis (Real-time and Accelerated Stability Testing)
LC-MS analysis
Capillary Electrophoresis (CE)
Peptide Mass Fingerprinting (PMF)
Biological activity and binding studies using enzymatic assays, ELISA, BIAcore etc.
Endotoxin analysis
Host Cell Protein (HCP) and Host Cell DNA (HCD) analysis

Protein Stability Analysis

Real-time and accelerated stability testing
Long-term stability studies

Protein Conjugation and Labeling

Protein labeling/conjugation (HRP, biotin, Ruthenium, fluorescent labels etc.)
Conjugation of resin with specific protein/peptide

Crystallization Screening

Multi-dimensional optimization of crystallization conditions using commercial and customized screens for a target protein or complex
Sitting drop, hanging drop, micro-batch (under oil) and seeding
Micro-seeding and macro-seeding

Crystal Hit Identification

Microscopic examination of drops, staining and mass spectromety of harvested crystals
Preliminary X-ray diffraction

Protein-protein and Protein-drug Complexes

Crystallization of protein-inhibitor/ligand complexes (co-crystallization)
Soaking of crystals in inhibitor/ligand solution

Data Collection and Refinement

Cry protectant screening
Complete X-ray data collection using rotating anode source
Synchrotron data collection at Australian synchrotron
Molecular replacement and structure refinement
Structure analysis and data presentation

Why Aurigene Protein Sciences and Structural Biology Services?

Multidisciplinary team of scientists

Scalability with high purity and exceptional quality

Processing complex molecules

Protein engineering for enhanced crystallizability

Synchrotron data collection

Virtual Tour

Hyderabad R and D Center

Bangalore R and D Center

Connect with our scientific experts for your drug discovery, development, and manufacturing needs

We understand that clear communication is essential to successful collaborations, and that's why we have a dedicated team that is always ready to help you. Whether you have questions about our services, want to discuss a potential partnership, or simply want to learn more about our company, we're here to help.

Our team of experts is dedicated to providing personalised solutions tailored to your unique needs. So, please don't hesitate to reach out to us. We look forward to hearing from you and helping you achieve your business goals.

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