PAMPA Assay
At Aurigene Pharmaceutical Services, our ADME services help understand the permeability of a drug by using Parallel Artificial Membrane Permeability (PAMPA) assay, an artificial lipid-infused assay used to measure the passive and transcellular permeability of a test compound. The advantage of using PAMPA assay over other assays is the capability to evaluate permeability over a large spectrum of pH ranges in a short period of time.
Advantages of our Parallel Artificial Membrane Permeability (PAMPA) assay
- Quick turn around time
- Broad pH range
- High (Dimethyl sulfoxide) DMSO content
- High throughput (96-well plate assay)
PAMPA eliminates the interference of active transporters or efflux systems present in other assays, allowing the compound to be measured based on permeability data alone.
| Test Article Concentration | 10µM |
| Number of Replicates | 3 |
| Membrane Composition | Precoated (lipid-infused artificial membrane) |
| Incubation Time | 5 hours |
| Temperature | 25 °C |
| Test Article Requirements | 50µL (10mM DMSO stock) |
| Integrity Marker | Not applicable (Standard quality controls) |
| Analysis Method | LCMS-MS |
| Data Delivery | 5 working days |
PAMPA assay provides researchers a quick, economical method of evaluating the permeability of New Chemical Entities (NCEs).
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