Analytical services are used to identify, isolate, or quantify molecules and to characterize their physical properties. We provide physiochemical and biological characterization services.
Backed by our strong protein chemistry background, we enable ‘Finger-Print’ protein structure and functional characterization for proteins from naked proteins to hyper-glycosylated or derivatized proteins.
Our biological characterization capability enables a sound understanding of the biological mechanism of action of the product. We can support a wide variety of biological assays including, cell-proliferation/killing, binding affinity, cell signaling, and viability or endpoint assays using both transformed cell lines and primary cells.
Physiochemical characterization
We provide physiochemical characterization services ranging from protein structure confirmation to amino acid content detection. We also quantify free cysteines and detect product impurities such as glycation and deamidation.
The ability is based on a diverse platform of instruments and technologies supported by a fully-trained technical staff:
- 21 CFR part 11 and GAMP compliant system-based labs
- A comprehensive instrumentation infrastructure to support orthogonal confirmation of product parameters
- Experience across diverse protein expression systems
Catalog:
- Primary structure: Intact mass, fragmented mass, and Pep map
- Higher order structure: Secondary and tertiary structure, melting point, disulfide linkage, and free thiol
- Post-translational modification: Oligosaccharide profiling, oxidation, deamidation, and cleavage
- Product/process - related impurities: Size and charge variant analysis
- Technology: Mass Spectrometry, HPLC, UPLC, spectroscopy, circular dichroism, fluorometry, DSC DLS, SEC MALS, ion chromatography, CEIEF, CESDS, and Solo VpE
- Protein: Mab, Fab, fusion protein, cytokine, enzyme, hormone, hyperglycosylated proteins, and derivatized proteins
Biological characterization
Our capability in luminescence and fluorescence-based assays and flow cytometry enables a wider reach of biological characterization. In addition, we also develop bio-assays for clinical sample analysis and immunogenicity assessment:
- Development of bioassays and cell-based assay employing primary and derived cell lines
- Expertise in luminescence, fluorescence, and flow cytometry-based assay
- Wide variety of cell signaling and endpoint assays – ADCC, CDC, and proliferation
- Ability to handle clinical samples
Catalog:
- Signaling: GPCR, calcium release, kinase, and phosphorylation
- Binding: Direct/indirect ELISA, competition ELISA, and surface plasmon resonance (Biacore)
- Viability: ADCC, CDC, and proliferation
- PK/PD: Serum drug concentration, immunogenicity, and neutralizing antibody
- Technology: Luminescence and fluorescence-based methods, multicolor flow cytometry, microscopy, and primary cell culture
- Protein: Mab, Fab, fusion protein, cytokine, enzyme, hormone, hyperglycosylated proteins, and derivatized proteins
Why Aurigene Analytical Services?
ALCOA+ driven quality systems
Diverse platform of instruments and technologies
Integrated approach to safety and quality management systems
In-house capability across the value chain with a trained scientific staff
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Hyderabad R and D Center
Bangalore R and D Center
Connect with our scientific experts for your drug discovery, development, and manufacturing needs
We understand that clear communication is essential to successful collaborations, and that's why we have a dedicated team that is always ready to help you. Whether you have questions about our services, want to discuss a potential partnership, or simply want to learn more about our company, we're here to help.
Our team of experts is dedicated to providing personalised solutions tailored to your unique needs. So, please don't hesitate to reach out to us. We look forward to hearing from you and helping you achieve your business goals.
Methoxy Polyethylene Glycol (m-PEGs)
Aurigene Pharmaceutical Services is a leader in the synthesis of activated MethoxyPolyethyleneGlycol(m-PEGs), With a comprehensive product range and customized services. ...
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Base mediated spirocyclization of quinazoline: one-step synthesis of spiro-isoindolinone dihydroquinazolinones
2020
A novel approach for the spiro-isoindolinone dihydroquinazolinones has been demonstrated from 2- aminobenzamide and 2-cyanomethyl benzoate in the presence of KHMDS as a base to get moderate yields. The reaction has been screened in various bases followed by solvents and a gram scale reaction has also been executed under the given conditions. Based on the controll...
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Ultrasound assisted rapid synthesis of mefenamic acid based indole derivatives under ligand free Cu-catalysis: Their pharmacological evaluation
2005
An improved and rapid synthesis of mefenamic acid based indole derivatives has been achieved via the ligand free Cu-catalyzed coupling-cyclization method under ultrasound irradiation. This simple, straightforward and inexpensive one-pot method involved the reaction of a terminal alkyne derived from mefenamic acid with 2- iodosulfanilides in the presence of CuI ...
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Formal synthesis of Cladospolide C & epi-Cladospolide C using R- (þ)-g-valerolactone as a chiral synthon
2005
The formal synthesis of Cladospolide-C and its analog is achieved by using enantiopure (R)-g evalerolactone 10. The significant points of this synthesis are the stereoselective dihydroxylation of a, bunsaturated ester 16 using Sharpless protocol, Wittig olefination of g evalerolactol 6 with triphenylphosphonium iodide salt 7, one pot selective oxidation ...
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Amberlite-15 promoted an unprecedented aza Michael rearrangement for one pot synthesis of dihydroquinazolinone compounds
2005
A new one pot multicomponent annulation strategy for the synthesis of various dihydroquinazolinone compounds has been developed using Amberlite-15 as a catalyst, giving good to moderate yields. In this reaction the substrate scope for amines and aldehydes was also investigated. The reaction has been checked on a large scale ...
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