We support and offer a diverse range of services in the areas of:
Recombinant DNA technology Antibody gene sequencing Mutagenesis Library generation Plasmid DNA preparation Microbial genome engineering Gene expression analysis and IVT mRNA synthesis
Infrastructure and facilities
Our state-of-the-art laboratory equipped with high-end instrumentation and the experienced IT and engineering teams support discovery and development services under biology capabilities.
Real-Time PCR, Gradient PCR instruments, GeneAmp PCR system Gel documentation systems Nanodrop spectrophotometer Pulsed field electrophoresis Benchtop fermenters Biosafety cabinets- BSL I & II Static incubators and shakers Wave bioreactor Flow cytometer LiCoR imaging system Biorad molecular imager Nikon Eclipse E800- inverted light microscope Shimadzu UV spectrophotometer Beckman DU 640 spectrophotometer Perkin Elmer Victor X5 2030 multilabel reader Biotek microplate reader Photomultiplier detection system
Recombinant DNA technology
Cloning and vector generation:
Gene cloning service at APSL supports customized generation of recombinant constructs by various methods for generation of antibodies, enzymes, and reagent proteins:
- Ligation dependent and independent cloning
- Recombination based high throughput cloning
- De novo cloning (synthetic gene fragments, PCR, and cDNA based)
- Customized vector construction based on client requirement
Plasmid DNA preparation:
Plasmid DNA purification service caters to both small research facilities as well as large-scale manufacturing for biotech and pharmaceutical companies:
- Research grade plasmid at small scale suitable for cloning, mutagenesis, microbial transformation, and protein production
- High-quality industrial grade plasmid suitable for :
- protein
- antibody production
- stable cell line generation in mammalian cells
- bacmid generation for protein expression in insect cells
- viral packaging
- in vitro mRNA synthesis
- vaccine
- gene therapy studies, and
- animal immunization
- Process development for customized plasmid propagation and purification
Mutagenesis:
Site-directed mutagenesis service enables life science researchers by creating specific DNA mutations for studying gene regulation, DNA-protein interactions, protein structure/function relationship, enzyme function, and novel recombinant proteins:
- Point mutation, insertions, deletion, or gene fragment replacements
- Customized mutagenesis requirements such as random and site-directed mutagenesis and mutant library generation.
- Gene shuffling, combinatorial mutagenesis, alanine scanning, and site saturation mutagenesis
Antibody gene sequencing and reformatting
Antibody sequence information from hybridoma is essential for protection, preservation, and optimization of monoclonal antibodies (mAbs). Antibody gene sequencing and reformatting at APSL is a one-stop solution for recombinant expression and production of antibodies:
- RACE (Rapid Amplification of cDNA Ends) using template switching adapter ligation
- Degenerate oligos
- De novo antibody sequencing and analysis using LC-MS/MS
- Reformatting antibody gene sequences into various other production formats like bispecifics, mAb, scFv, Fab, F (ab)'2, and VHH/nanobody
- Antibody gene sequencing for mouse, rat, rabbit and other species.
Strain engineering
Experience in genome scale modification of bacterial and yeast strains for production of recombinant proteins, platform chemicals, and metabolic engineering:
- BSL-1 and BSL-2 microbes
- Gene knock out and knock-in using homologous recombination based methods
- Characterization of engineered strains using microbiological methods, RT-PCR, and advanced analytics
- CRISPR-Cas9 based mutagenesis
Library generation and evaluation
Constructing cDNA libraries, phage libraries, and yeast libraries along with provisions for high throughput screening and functional characterization.
Gene expression analysis
Regulation and expression of genes at the transcriptional and translational stages play a crucial role in determining gene function. Scientists at APSL carry out routine expression studies by several methods such as:
- Quantitative real time PCR
- ELISA in various formats
- Flow cytometry
- Western blotting
- Mass spectrometry
Residual protein, DNA, and Mycoplasma detection
Residual host-cell proteins (HCP) and host-cell DNA (HCD) are impurities that remain in recombinant bio therapeutics. We ensure that these impurities and contaminants are detected and reduced to levels below those guided by regulatory agencies:
- Quantitative real-time PCR
- ELISA
- SPR (Biacore)
- Mycoplasma test
Why Aurigene Molecular Biology Services?
State-of-the-art facilities with high-end instrumentation
Wide range of tools under one umbrella
Small-scale and large-scale manufacturing
Customized generation of recombinant constructs
Fast and innovative solutions
One-stop solution for recombinant expression and production of antibodies
Virtual Tour
Hyderabad R and D Center
Bangalore R and D Center
Connect with our scientific experts for your drug discovery, development, and manufacturing needs
We understand that clear communication is essential to successful collaborations, and that's why we have a dedicated team that is always ready to help you. Whether you have questions about our services, want to discuss a potential partnership, or simply want to learn more about our company, we're here to help.
Our team of experts is dedicated to providing personalised solutions tailored to your unique needs. So, please don't hesitate to reach out to us. We look forward to hearing from you and helping you achieve your business goals.
Methoxy Polyethylene Glycol (m-PEGs)
Aurigene Pharmaceutical Services is a leader in the synthesis of activated MethoxyPolyethyleneGlycol(m-PEGs), With a comprehensive product range and customized services. ...
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Base mediated spirocyclization of quinazoline: one-step synthesis of spiro-isoindolinone dihydroquinazolinones
2020
A novel approach for the spiro-isoindolinone dihydroquinazolinones has been demonstrated from 2- aminobenzamide and 2-cyanomethyl benzoate in the presence of KHMDS as a base to get moderate yields. The reaction has been screened in various bases followed by solvents and a gram scale reaction has also been executed under the given conditions. Based on the controll...
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Ultrasound assisted rapid synthesis of mefenamic acid based indole derivatives under ligand free Cu-catalysis: Their pharmacological evaluation
2005
An improved and rapid synthesis of mefenamic acid based indole derivatives has been achieved via the ligand free Cu-catalyzed coupling-cyclization method under ultrasound irradiation. This simple, straightforward and inexpensive one-pot method involved the reaction of a terminal alkyne derived from mefenamic acid with 2- iodosulfanilides in the presence of CuI ...
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Formal synthesis of Cladospolide C & epi-Cladospolide C using R- (þ)-g-valerolactone as a chiral synthon
2005
The formal synthesis of Cladospolide-C and its analog is achieved by using enantiopure (R)-g evalerolactone 10. The significant points of this synthesis are the stereoselective dihydroxylation of a, bunsaturated ester 16 using Sharpless protocol, Wittig olefination of g evalerolactol 6 with triphenylphosphonium iodide salt 7, one pot selective oxidation ...
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Amberlite-15 promoted an unprecedented aza Michael rearrangement for one pot synthesis of dihydroquinazolinone compounds
2005
A new one pot multicomponent annulation strategy for the synthesis of various dihydroquinazolinone compounds has been developed using Amberlite-15 as a catalyst, giving good to moderate yields. In this reaction the substrate scope for amines and aldehydes was also investigated. The reaction has been checked on a large scale ...
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