Our cell engineering services cater to both tool/reagent and biological product development requirements. We have strong expertise to generate recombinant/stable cell lines for complex and wide protein class targets such as:
RTKs GPCRs Ion channels Immune modulation ligands/receptors Fc-gamma receptors GPI-anchored TNF-receptor superfamily Cell adhesion molecules Transporters Co-receptors and Reporter genes
We are well-versed in working with different cellular backgrounds such as established cancer cell lines and primary cells. Hard-to-transfect cell lines required for binding studies, functional assays, immunization in vitro/in vivo screening, and efficacy evaluation applications are successfully engineered to achieve the desired attributes.
For biologic programs, we have experience of product development for mAbs, immune-fusion molecules, and cytokines. The typical activities include:
Transfection Selection Limiting dilution Media/feed screening Cell line characterization Research cell banking and Protein production
We have the experience of multiple production platforms.
Cell lines
- Membrane-bound receptor cell lines with expression calibrated clones (low, medium, and high)
- Receptor density measured and clones selected as per end-use requirement
- Knock-In (KI), Knock-Down (KD) and Knock-Out (KO) cell lines using siRNA / shRNA & CRISPR/Cas9 or TALEN technologies
- Single, double, or multiple gene KO / KD in primary and established cell lines
- Inducible cell line using Tet / Cumate inducible system
- Reporter cell lines (Fluorescent protein / Luciferase)
- Orthotopic cell line for in vivo xenograft and imaging applications
- Production cell lines using commercially available systems such as Freedom CHO-S/CHO DG44/CHOZN, and in-house developed lentiviral system
- SARS-CoV-2 pseudo virus generation and neutralization assay
Vector systems
pcDNA series, pLVX and pGIPZ / pTRIPZ lentiviral vector series, pCHO1.0, pOptivec, pGL, and in-house modified vector systems
Transfection methods
Standard (lipid/nanoparticle), electroporation, transposon, and lentiviral transduction
Screening
Flow cytometry, Western blot, PA-HPLC, ELISA, Octet, and ClonePix
Quality control
Thaw test, mycoplasma, clonal stability, receptor density estimation, SE-HPLC and LC-MS analysis
Why Aurigene Cell Line Engineering Services?
Strong framework data recording and traceability
Recombinant cell lines for wide protein classes
In-house lentiviral platform for difficult to express reagent proteins
Extensive experience working with various vector / cell line systems
Virtual Tour
Hyderabad R and D Center
Bangalore R and D Center
Connect with our scientific experts for your drug discovery, development, and manufacturing needs
We understand that clear communication is essential to successful collaborations, and that's why we have a dedicated team that is always ready to help you. Whether you have questions about our services, want to discuss a potential partnership, or simply want to learn more about our company, we're here to help.
Our team of experts is dedicated to providing personalised solutions tailored to your unique needs. So, please don't hesitate to reach out to us. We look forward to hearing from you and helping you achieve your business goals.
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A novel approach for the spiro-isoindolinone dihydroquinazolinones has been demonstrated from 2- aminobenzamide and 2-cyanomethyl benzoate in the presence of KHMDS as a base to get moderate yields. The reaction has been screened in various bases followed by solvents and a gram scale reaction has also been executed under the given conditions. Based on the controll...
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An improved and rapid synthesis of mefenamic acid based indole derivatives has been achieved via the ligand free Cu-catalyzed coupling-cyclization method under ultrasound irradiation. This simple, straightforward and inexpensive one-pot method involved the reaction of a terminal alkyne derived from mefenamic acid with 2- iodosulfanilides in the presence of CuI ...
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